Amplified detection of femtomolar DNA based on a one-to-few recognition reaction between DNA-Au conjugate and target DNA.

Citation:

Zhijuan Wang, Zhang, Juan , Zhu, Changfeng , Wu, Shixin , Mandler, Daniel , Marks, Robert S, and Zhang, Hua. . 2014. “Amplified Detection Of Femtomolar Dna Based On A One-To-Few Recognition Reaction Between Dna-Au Conjugate And Target Dna.”. Nanoscalenanoscale, 6, Pp. 3110 - 3115.

Abstract:

A sensitive electrochem. DNA biosensor based on the amplification of Au nanoparticles (AuNPs) has been developed. The AuNPs were modified with two types of signaling reporter DNAs, i.e. a methylene blue probe (MB-probe 2-SH) and T10 with a methylene blue signaling mol. (MB-T10-SH), forming DNA-AuNP conjugates. The MB-probe 2-SH is complementary to the target DNA, while MB-T10-SH is not. The presence of MB-T10-SH reduces the cross-reaction between target DNA and MB-probe 2-SH on the AuNPs, resulting in increased sensitivity of the biosensor. In our assay, the DNA sensor is fabricated by immobilizing a capture probe on the surface of the Au electrode, which then hybridizes with the corresponding target DNA, and further hybridizes with a DNA-Au conjugate. The signal of MB is measured by differential pulse voltammetry, while the DNA-Au conjugate enables the detection of target DNA in the linear range of 10-13 to 10-8 M with the detection limit as low as 50 fM. [on SciFinder(R)]