Label-free femtomolar cancer biomarker detection in human serum using graphene-coated surface plasmon resonance chips.

Citation:

Lijie He, Pagneux, Quentin , Larroulet, Iban , Serrano, Aritz Yanguas, Pesquera, Amaia , Zurutuza, Amaia , Mandler, Daniel , Boukherroub, Rabah , and Szunerits, Sabine. . 2016. “Label-Free Femtomolar Cancer Biomarker Detection In Human Serum Using Graphene-Coated Surface Plasmon Resonance Chips.”. Biosens. Bioelectron.biosensors & Bioelectronics, Pp. Ahead of Print.

Abstract:

Sensitive and selective detection of cancer biomarkers is vital for the successful diagnosis of early stage cancer and follow-up treatment. Surface Plasmon Resonance (SPR) in combination with different amplification strategies is one of the anal. approaches allowing the screening of protein biomarkers in serum. Here we describe the development of a point-of-care sensor for the detection of folic acid protein (FAP) using graphene-based SPR chips. The exceptional properties of CVD graphene were exploited to construct a highly sensitive and selective SPR chip for folate biomarker sensing in serum. The specific recognition of FAP is based on the interaction between folic acid receptors integrated through π-stacking on the graphene coated SPR chip and the FAP analyte in serum. A simple post-adsorption of human serum:bovine serum albumin (HS:BSA) mixts. onto the folic acid modified sensor resulted in a highly anti-fouling interface, while keeping the sensing capabilities for folate biomarkers. This sensor allowed femtomolar (fM) detection of FAP, a detection limit well adapted and promising for quant. clin. anal. [on SciFinder(R)]