Citation:
Lijie He, Pagneux, Quentin , Larroulet, Iban , Serrano, Aritz Yanguas, Pesquera, Amaia , Zurutuza, Amaia , Mandler, Daniel , Boukherroub, Rabah , and Szunerits, Sabine . 2017.
“Label-Free Femtomolar Cancer Biomarker Detection In Human Serum Using Graphene-Coated Surface Plasmon Resonance Chips”. Biosensors & Bioelectronics, 89, Pp. 606-611. doi:10.1016/j.bios.2016.01.076.
Abstract:
Sensitive and selective detection of cancer biomarkers is vital for the successful diagnosis of early stage cancer and follow-up treatment. Surface Plasmon Resonance (SPR) in combination with different amplification strategies is one of the analytical approaches allowing the screening of protein biomarkers in serum. Here we describe the development of a point-of-care sensor for the detection of folic acid protein (FAP) using graphene-based SPR chips. The exceptional properties of CVD graphene were exploited to construct a highly sensitive and selective SPR chip for folate biomarker sensing in serum. The specific recognition of FAP is based on the interaction between folic acid receptors integrated through pi-stacking on the graphene coated SPR chip and the FAP analyte in serum. A simple post-adsorption of human serum:bovine serum albumin (HS:BSA) mixtures onto the folic acid modified sensor resulted in a highly anti-fouling interface, while keeping the sensing capabilities for folate biomarkers. This sensor allowed femtomolar (fM) detection of FAP, a detection limit well adapted and promising for quantitative clinical analysis. (C) 2016 Elsevier B.V. All rights reserved.
